Each volunteer must be: (i) male or female, 50 to 75 years old, undergoing routine colonoscopy, (ii) without any history of CRC or IBD, (iii) without having antibiotics or suffering from gastroenteritis for the past month, (iv) without requiring blood thinning medication 2 days before and 2 days after the …
Visit prior to colonoscopy: The volunteer brings in doctor’s office before colonoscopy (a) fecal sample on ice, (b) full blood test exams; and (c) the filled in QUESTIONAIRE; the doctor goes through to make sure the volunteers added all the correct information. Personal data will not be distributed to the …
3’mRNA sequencing quantification by Next Generation Sequencing will be achieved using the QuantSeq 3′ mRNA-Seq Library Prep Kit (Lexogen). QuantSeq is a highly strand-specific sequencing approach that reduces data analysis time and enables a higher level of multiplexing per run compared to standard mRNA sequencing method. Statistical power & transcriptomics …
Bacterial abundance relative to the host tissue via qPCR: To target 16S rDNA genes we are using universal (pan-bacterial) primers & primers specific to the following genera that dominate the human colon: a) Firmicutes b) Bacteroides c) Proteobacteria d) Actinobacteria. To normalize bacterial load to host tissue sample, one reference …
