The Cyprus Intestinal Health Study

Molecular colonoscopy is a project aiming to predict the risk for developing Colorectal Cancer based on individualized multi-parametric and multi-omic analysis.

Molecular Colonoscopy & Multi-omics.

Colorectal Cancer (CRC) & Inflammatory Bowel Disease (IBD)

Human Genes, Microbes and Environment in CRC and IBD

Model Systems of CRC and IBD for “treatable” biomarker identification

Molecular Colonoscopy & Multi-omics.

We will implement molecular colonoscopy, a cutting edge molecular risk assessment tool, for estimating the risk of healthy-appearing individuals for colorectal neoplasia. Using this tool,...

Model Systems of CRC and IBD for “treatable” biomarker identification

The complex nature of CRC integrating genetic, epigenetic, environmental and microbial cues underscores the need for a holistic perspective and suggests that assessing these factors...

Human Genes, Microbes and Environment in CRC and IBD

Host-microbe interactions control colonic dysbiosis, aberrant immune responses and intestinal epithelium damage, potentially predisposing for colorectal cancer (CRC). Most studies on CRC focus on tumor...

Colorectal Cancer (CRC) & Inflammatory Bowel Disease (IBD)

Among cancers that affect both men and women, colorectal cancer (CRC) is the second leading cause of death in the United States and Europe. In...

Molecular Colonoscopy & Multi-omics.

We will implement molecular colonoscopy, a cutting edge molecular risk assessment tool, for estimating the risk of healthy-appearing individuals for colorectal neoplasia. Using this tool,...

Model Systems of CRC and IBD for “treatable” biomarker identification

The complex nature of CRC integrating genetic, epigenetic, environmental and microbial cues underscores the need for a holistic perspective and suggests that assessing these factors...

Human Genes, Microbes and Environment in CRC and IBD

Colorectal Cancer (CRC) & Inflammatory Bowel Disease (IBD)

Among cancers that affect both men and women, colorectal cancer (CRC) is the second leading cause of death in the United States and Europe. In...

Enrollment & Disease Criteria

Each volunteer must be: (i) male or female, 50 to 75 years old, undergoing routine colonoscopy, (ii) without any history of CRC or IBD, (iii)...

Sample Collection

Visit prior to colonoscopy: The volunteer brings in doctor’s office before colonoscopy (a) fecal sample on ice, (b) full blood test exams; and (c) the...

QuantSeq

3’mRNA sequencing quantification by Next Generation Sequencing will be achieved using the QuantSeq 3' mRNA-Seq Library Prep Kit (Lexogen). QuantSeq is a highly strand-specific sequencing...

Bacteria quantification

Bacterial abundance relative to the host tissue via qPCR: To target 16S rDNA genes we are using universal (pan-bacterial) primers & primers specific to the...